Affinity Measurement of Antibodies in Allergy Diagnosis

Abstract

u SPRi allows a multiplex monitoring of the complex antibody response to the major allergens of an allergenic source u SPRi monitoring is faster, label-free and more informative than ELISA, but less sensitive u The avidity of the antibody response may be evaluated and this evaluation is reproducible Conclusion The biological diagnosis of type I hypersensitivity reactions is based on the quantification of specific IgEs. However, the IgE titer is not always strongly related to the clinical symptoms or predictive of the evolution of the disease. The specificity and affinity of antibodies of other isotypes may contribute to the allergic status of the patients. The aim of this work was to develop a method that simultaneously detects the complex antibody response to various allergens and measures the avidity of the antibodies directed to each allergen (Cf Journal of immunological Methods, Volume 405, March 2014, Pages 23–28). We developed a tool to detect and measure the avidity of the various antibodies recognizing the major allergens of an allergenic source. Surface Plasmon Resonance imaging (SPRi) is a sensitive label-free method to detect and visualize biomolecular interactions. Our configuration allows the use of a pattern of various immobilized molecules and simultaneously follows up the kinetic curves of each spotted molecule. Introduction