Affinity Isolation of Desired Restriction Fragment from Human Genome Using Double-duplex Invasion of Biotin-bound Pseudo-complementary PNA

Abstract

A predetermined fragment in the human genome was successfully isolated by affinity separation using biotin–streptavidin interaction. The whole genome from human cells was first digested by restriction enzymes and then treated with a pair of pseudo-complementary peptide nucleic acids (pcPNAs). A biotin was covalently bound to either of the pcPNAs. With the use of streptavidin-coupled resin, the targeted fragment involving the invasion site of the pcPNAs was selectively picked up from the genomic digests in high yields.