Afferent and efferent connections of the caudolateral neostriatum in the pigeon (Columba livia): a retro- and anterograde pathway tracing study.

Abstract

The avian caudolateral neostriatum (NCL) was first identified on the basis of its dense dopaminergic innervation. This fact and data from lesion studies have led to the notion that NCL might be the avian equivalent of prefrontal cortex (PFC). A key feature of the PFC is the ability to integrate information from all modalities needed for the generation of motor plans. By using antero- and retrograde pathway tracing techniques, we investigated the organization of sensory afferents to the NCL and the connections with limbic and somatomotor centers in the basal ganglia and archistriatum. Data from all tracing experiments were compared with the distribution of tyrosine-hydroxylase (TH)-immunoreactive fibers, serving as a marker of dopaminergic innervation. The results show that NCL is reciprocally connected with the secondary sensory areas of all modalities and with at least two parasensory areas. Retrograde tracing also demonstrated further afferents from the deep layers of the Wulst and from the frontolateral neostriatum as well as the sources of thalamic input. Efferents of NCL project onto parts of the avian basal ganglia considered to serve somatomotor or limbic functions. Projections to the archistriatum are mainly directed to the somatomotor part of the intermediate archistriatum. In addition, cells in caudal NCL were found to be connected with the ventral and posterior archistriatum, which are considered avian equivalents of mammalian amygdala. All afferents and projection neurons were confined to the plexus of densest TH innervation. Our results show that the NCL is positioned to amalgamate information from all modalities and to exert control over limbic and somatomotor areas. This organization might comprise the neural basis for such complex behaviours as working memory or spatial orientation.