AFB1 Induced Transcriptional Regulation Related to Apoptosis and Lipid Metabolism in Liver of Chicken.

Xueqin Liu,Huadong Yin,Mingyao Yang,Deying Yang,Feng Chen,Qing Zhu,Tao Wang,Yan Li,Qingyong Ni,Xiaolan Fan,Bo Zeng,Shailendra Kumar Mishra,Xiaoling Zhao,Yan Wang,Mingwang Zhang,Zhongxian Xu,Diyan Li

doi:10.3390/toxins12050290

Xueqin Liu, Huadong Yin + Show 15 more

Open Access

https://doi.org/10.3390/toxins12050290

Copy DOI

Abstract

Aflatoxin B1 (AFB1) leads to a major risk to poultry and its residues in meat products can also pose serious threat to human health. In this study, after feeding 165-day-old Roman laying hens for 35 days, the toxic effects of aflatoxin B1 at different concentrations were evaluated. The purpose of this study was to explore the mechanism of liver toxicosis responses to AFB1. We found that highly toxic group exposure resulted in liver fat deposition, increased interstitial space, and hepatocyte apoptosis in laying hens. Furthermore, a total of 164 differentially expressed lnRNAs and 186 differentially expressed genes were found to be highly correlated (Pearson Correlation Coefficient > 0.80, p-value < 0.05) by sequencing the transcriptome of control (CB) and highly toxic group (TB3) chickens. We also identify 29 differentially expressed genes and 19 miRNAs that have targeted regulatory relationships. Based on the liver cell apoptosis and fatty liver syndrome that this research focused on, we found that the highly toxic AFB1 led to dysregulation of the expression of PPARG and BCL6. They are cis-regulated by TU10057 and TU45776, respectively. PPARG was the target gene of gga-miR-301a-3p, gga-miR-301b-3p, and BCL6 was the target gene of gga-miR-190a-3p. In summary, highly toxic AFB1 affects the expression levels of protein-coding genes and miRNAs in the liver of Roman layer hens, as well as the expression level of long non-coding RNA in the liver, which upregulates the expression of PPARG and downregulates the expression of Bcl-6. Our study provides information on possible genetic regulatory networks in AFB1-induced hepatic fat deposition and hepatocyte apoptosis.

Highlights

Aflatoxin B1 (AFB1) is a secondary fungal metabolite product widely found in many foodstuffs and considered a public health concern worldwide due to its carcinogenic property [1]
This study indicated that Long non-coding RNA (lncRNA) may become a diagnostic marker and therapeutic target for cancer. miRNAs are 19-24 nucleotide regulatory small RNAs that generally modulate gene expression through translational repression or by causing the degeneration and degradation of target mRNAs to function in many aspects of biological function [27]
We found that peroxisome proliferator-activated receptor gamma (PPARG) is simultaneously regulated by one lncRNA (TU10057) and two miRNAs

Summary

Introduction

Aflatoxin B1 (AFB1) is a secondary fungal metabolite product widely found in many foodstuffs and considered a public health concern worldwide due to its carcinogenic property [1]. AFB1 is the most important hepatotoxic, mutagenic and universal food-borne mycotoxin [2]. Exposure to symptoms and biomarkers, and methods for reducing aflatoxins have been extensively studied [3,4]. Previous studies have been reported that after intake of AFB1, the liver shows obvious fat deposition [5,6]. Other symptoms of AFB1, such as immune damage, oxidative stress, apoptosis, and tumorigenesis, have been gradually reported [7,8,9]

Objectives

Methods

Results

Discussion

Conclusion