Abstract
AF166 and AI179 antibodies against the Myc tag recognize a Myc-tagged human TAC protein by immunofluorescence in paraformaldehyde-fixed HeLa cells; AF372, AF373 and AI831 do not.
Highlights
The Myc tag is a short hydrophilic peptide derived from the human proto-oncogene c-myc protein (Uniprot #P01106), extensively used for detection of tagged proteins using anti-Myc antibodies
The Myc-tagged TAC protein is expected to be mostly present at the cell surface
After 3 washes (10 min) with PBS + 0.2% (w/v) BSA (PBS-BSA), cells were incubated for 30 min in PBS-BSA with secondary goat anti-mouse IgG conjugated to AlexaFluor-647 (1:300, Molecular Probes, #A21235) and, when AJ519 was used for co-localization, goat anti-rabbit IgG conjugated to AlexaFluor-488 (1:300, Molecular Probes #A11034)
Summary
The Myc tag is a short hydrophilic peptide derived from the human proto-oncogene c-myc protein (Uniprot #P01106), extensively used for detection of tagged proteins using anti-Myc antibodies. Facility (www.unige.ch/medecine/antibodies/) as miniantibodies with the antigen-binding scFv fused to a mouse Antigen: HeLa cells (growing in DMEM GlutaMAXTM, Gibco #31966; supplemented with 8% Fetal Bovine Serum, Gibco #10270) cultured on glass coverslips (Menzel-Gläser, 22x22 mm) and transiently transfected 2 days before the experiment with a Myc-tagged TAC protein (Uniprot #P01589), were used to detect the peptide tag.
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