Abstract
Inhalation of dry powder synthetic lung surfactant may assist spontaneous breathing by providing noninvasive surfactant therapy for premature infants supported with nasal continuous positive airway pressure. Surfactant was formulated using spray-drying with different phospholipid compositions (70 or 80 total weight% and 7:3 or 4:1 DPPC:POPG ratios), a surfactant protein B peptide analog (KL4, Super Mini-B, or B-YL), and Lactose or Trehalose as excipient. KL4 surfactant underperformed on initial adsorption and surface activity at captive bubble surfactometry. Spray-drying had no effect on the chemical composition of Super Mini-B and B-YL peptides and surfactant with these peptides had excellent surface activity with particle sizes and fine particle fractions that were well within the margins for respiratory particles and similar solid-state properties. Prolonged exposure of the dry powder surfactants with lactose as excipient to 40 °C and 75% humidity negatively affected hysteresis during dynamic cycling in the captive bubble surfactometer. Dry powder synthetic lung surfactants with 70% phospholipids (DPPC and POPG at a 7:3 ratio), 25% trehalose and 3% of SMB or B-YL showed excellent surface activity and good short-term stability, thereby qualifying them for potential clinical use in premature infants.
Highlights
Inhalation of dry powder synthetic lung surfactant may assist spontaneous breathing by providing noninvasive surfactant therapy for premature infants supported with nasal continuous positive airway pressure
The first series of six dry powder synthetic lung surfactants focused on phospholipid ratio (DPPC:POPG ratio of 7:3 or 4:1) and quantity (70 or 80 wt%) and SP-B peptide mimic selection (3 weight% of KL4 or SMB), all powders contained lactose as sugar excipient
Initial adsorption (IA) of SMB surfactants was significantly better than that of the KL4 surfactants (p < 0.02) (Fig. 2), but there were no differences in post-expansion adsorption (PEA)
Summary
Inhalation of dry powder synthetic lung surfactant may assist spontaneous breathing by providing noninvasive surfactant therapy for premature infants supported with nasal continuous positive airway pressure. The primary aim of our study was to develop an advanced dry powder (DP) synthetic lung surfactant for aerosol delivery to preterm infants with respiratory distress syndrome who need noninvasive respiratory support with nasal (continuous positive airway pressure) CPAP in a low technical setting. This surfactant would need to replicate the surface activity of clinically used liquid animal-derived lung surfactant and generate a physically and Scientific Reports | (2021) 11:16439. Lactose is an approved excipient for inhaled pharmaceutical products, trehalose was included in the excipient screening procedure as this disaccharide protects labile macromolecules and lipid m embranes[13]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
