Aeromycology: studies of fungi in aeroplankton

Abstract

Air is a natural environment for spores of many genera and species of fungi. Despite its small size and a significant dispersion they have a great impact on human health and different areas of our activities, such as agricultural production. The study on spores of fungi that belong to aeroplankton or bioaerosole is called aeromycology. The most frequent fungi present in the air are Cladosporium and Alternaria species. Their numbers are abundant regardless of latitude and height above the sea level and above the ground. They mostly originate from agricultural environment. Other frequently listed species of fungi, whose spores are present in the air include of Aspergillus, Penicillium, Fusarium, Sclerotinia and Ganoderma. The concentration of spores in the air strongly depends on the abundance of their formation during the studied period. This in turn relates to geobotanical region, vegetation, degree of urbanization, climatic conditions, season, current weather, wind force and direction, local microclimate, and many other factors. Changes in humidity affect the concentration of different types of fungal spores. In general they are divided to ‘dry’ (Alternaria, Cladosporium, Puccinia, Ustilago, Melampsora, Epicoccum, Drechslera) and ‘wet’ (Didymella, Fusarium, Ganoderma, Gliocladium, Leptosphaeria, Verticillium). Study of the composition of species and genera are being done using different types of spore samplers, mostly volumetric instruments. Visual identification is based on colony morphology of the fungus and the shape and size of spores. The identification at the species level is possible with molecular tools. Methods based on DNA/RNA amplification are very sensitive and accurate. They allow the identification below the species level, e.g. chemotypes, mating types or isolates with genes or alleles of interest. Aerobiological monitoring is widely used in the epidemiology of human diseases (inhalant allergies) and infections of arable crops (decision support systems for the protection of cultivated plants). Aeromycology is interconnected with such diverse areas as industrial aerobiology, bioterrorism, ecology, climatology or even speleology and cultural heritage.