Aerobic granulation strategy for bioaugmentation of a sequencing batch reactor (SBR) treating high strength pyridine wastewater

Abstract

Aerobic granules were successfully cultivated in a sequencing batch reactor (SBR), using a single bacterial strain Rhizobium sp. NJUST18 as the inoculum. NJUST18 presented as both a good pyridine degrader and an efficient autoaggregator. Stable granules with diameter of 0.5–1mm, sludge volume index of 25.6±3.6mL g−1 and settling velocity of 37.2±2.7mh−1, were formed in SBR following 120-day cultivation. These granules exhibited excellent pyridine degradation performance, with maximum volumetric degradation rate (Vmax) varied between 1164.5mgL−1h−1 and 1867.4mgL−1h−1. High-throughput sequencing analysis exhibited a large shift in microbial community structure, since the SBR was operated under open condition. Paracoccus and Comamonas were found to be the most predominant species in the aerobic granule system after the system had stabilized. The initially inoculated Rhizobium sp. lost its dominance during aerobic granulation. However, the inoculation of Rhizobium sp. played a key role in the start-up process of this bioaugmentation system. This study demonstrated that, in addition to the hydraulic selection pressure during settling and effluent discharge, the selection of aggregating bacterial inocula is equally important for the formation of the aerobic granule.