Aerobic exercise training and dehydroepiandrosterone administration increase testicular sex steroid hormones and enhance reproductive function in high-sucrose-induced obese rats

Abstract

This study assessed the effects of chronic dehydroepiandrosterone (DHEA) administration and exercise training on testicular sex steroid hormone levels and reproductive function in high-sucrose induced obese rats. After 14 weeks of a high-sucrose diet, Wistar male rats were assigned randomly to the control, exercise training (running at 25 m/min for 1 h, 5 days/week), DHEA administration, and combined exercise training and DHEA administration groups (n = 7 each group). Six weeks of DHEA administration and/or exercise training significantly increased plasma concentrations of DHEA and 5α-dihydrotestosterone (DHT) and epididymis DHEA concentrations; however, the expression of steroidogenic enzymes, such as 3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, and 5α-reductase, did not change following any interventions. Procathepsin L expression, which involved sperm maturation, was significantly lower in the DHEA and combination groups, and glutathione peroxidase 4 (GPx4) expression, which plays a role in protecting sperms from oxidative stress, was significantly increased in the DHEA administration group. Additionally, exercise training and/or DHEA administration-induced increase in procathepsin L expressions were significantly correlated with the epididymis DHEA concentrations. These findings suggest that exercise training and/or DHEA administration-induced increase in epididymis DHEA concentration may improve impairment of reproductive function in high-sucrose obese rats. Additionally, exercise training and/or DHEA administration-induced increase in DHEA concentration may have a role in testicular-specific action, which included protective role from exercise-induced oxidant damage as well as contributed to the enhancement of sperm modification and maturation in obese rats.