Aerobic Exercise Enhances Behavior Features In Model Of Parkinson’S Disease Mice Via Pink1/parkin Pathway

Abstract

Oxidative stress in the brain of Parkinson's patients leads to impaired mitochondrial function, while exercise can improve mitochondrial function, but the mechanism is unclear. There are two mechanisms of mitochondrial function: autophagy and transport function. PURPOSE: To detect the expression of mitochondrial autophagy protein PINK1/Parkin in MPTP-induced Parkinson's (PD) mice by treadmill exercise, and to explore the effect of aerobic exercise on mitochondrial function. METHODS:32 C57BL/6J male mice were divided into 4 groups: Saline group (S), Saline+ Exercise group (SE), MPTP group (M), and MPTP + Exercise group (ME). M and ME mice were injected with MPTP to construct a PD model. SE and ME mice were subjected to 8 weeks treadmill training. Behavioral tests were performed after exercise; immunofluorescence and histochemistry, and Western Blot to detect molecular indicators. RESULTS: (1) The time of passing the balance beam, M group was longer than the S group (P<0.01), and the ME group was shorter than the M group (P<0.05)(Fig1A); In the forced swimming test (Fig1B)and the open field experiment (Fig1C\D)the mouse fast moving time , M group was shorter than the S group (P<0.05), while the ME group was longer than the M group(P<0.05). (2) The expression of α-Syn was up-regulated in the M group (P<0.01), but in the ME group was decreased after exercise (P<0.05) (Fig2). (3) The expression of Tyrosine hydroxylase (TH)(Fig3), TOM-40(Fig4) in group M was lower than that in group S (P<0.05), while ME group was higher than that M group (P<0.05). (4) The protein of Parkin and PINK1 were increased after exercise (P<0.05) (Fig5). CONCLUSION: Exercise can enhance the mitochondrial autophagy ability, improve the mitochondrial transport function, promotes behavior features in PD mice. Key words: aerobic exercise; mitochondria; autophagy; Parkinson’s disease Correspondent author: Dr. Jianshe Wei, M.D. & Ph.D., Email: [email protected]