Aerobic degradation of phthalic acid by Comamonas acidovoran Fy-1 and dimethyl phthalate ester by two reconstituted consortia from sewage sludge at high concentrations

Abstract

Microbial degradation of phthalic acid (PA) and dimethyl phthalate ester (DMPE) under aerobic conditions was investigated using a pure species of bacteria and two consortia from sewage sludge. Five morphologically distinct microorganisms were obtained in pure culture and identified, and tested for the capability of degrading phthalate and DMPE. Comamonas acidovorans strain Fy-1 showed the highest ability to degrade high concentrations of phthalate (2600 mg/l) within 48 h. Two reconstituted consortia of microorganisms, one comprising Pseudomonas fluorescens, P. aureofaciens and Sphingomonas paucimobilis, and the other of Xanthomonas maltophilia and S. paucimobilis, were effective in completely degrading DMPE (400 mg/l) in 48–96 h. The three-species consortium appeared to be more effective in the degradation of DMPE, and both consortia proceeded via formation of mono-methyl phthalate (MMP) and then phthalatic acid before mineralization. This study suggests that high concentrations of the endocrine-disrupting chemicals phthalate and DMPE can be mineralized in wastewater treatment systems by indigenous microorganisms.