Abstract
Trichloroethylene (TCE) is one of the most widely used solvent and have been poses a serious threats to the environment and human health. This work investigated to study the efficient biodegradation of synthetic TCE in a batch reactor using turkey litter compost in the concentration range of 25–500 mg L−1. The physical and biological properties are ensures that the compost has a great extent in biodegradation of hydrophobic compounds. Maximum TCE degradation rate was found to be 0.41 mg L-1 h-1 and more than 90% of removal efficiency was obtained up to the concentration of 300 mg L−1. High performance liquid chromatography was used to identify the major acid metabolites and confirmed the reported biochemical pathway; TCE was first hydrolyzed to dichloroacetyl chloride and then subsequently degraded to dichloroacetic acid and trichloroacetic acid and ultimately converted to carbon di-oxide and water. Furthermore, the degradation of TCE and their metabolites are well fitted with Aiba substrate inhibition and Monod model respectively. Based on biochemical test and 16S rDNA sequencing, Pseudomonas guguanensis NR135725 is an identified predominant strain in the TCE degraded culture. It is therefore concluded that a novel aerobic isolate involved in the degradation of TCE and their metabolites; in addition to offers a great potential application to use in continuous removal of chlorinated contaminants from industrial waste streams by effectively.
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