Aerobic biodegradation of sulfolane using Archaea and Pseudomonas strains

Abstract

AbstractBACKGROUNDSulfolane, an industrial solvent commonly used for sweetening natural gas, has recently emerged as a contaminant of concern in Alberta because of its widespread detection around many gas processing sites. In the work reported in this paper, the aerobic biodegradation of sulfolane by Pseudomonas strain or Archaea strain and mixed bacterial cultures was studied. The evaluation was furtherly conducted using Pseudomonas which had been acclimated to water contaminated with sulfolane. The impacts of co‐contaminant, initial sulfolane concentration and soil content on biodegradation of sulfolane using acclimated Pseudomonas were also investigated.RESULTSThe results showed that Pseudomonas degraded sulfolane at a rate of 2.03 mg L−1 h−1 while Archaea strain degraded sulfolane at a rate of 0.04 mg L−1 h−1. Pseudomonas and Archaea inoculated with aquifer sediments containing indigenous microbes achieved a higher sulfolane degradation rate. Acclimation of Pseudomonas to sulfolane environment sufficiently mitigated the lag period before the onset of the biodegradation process.CONCLUSIONSPseudomonas is a good candidate for aerobic biodegradation of sulfolane in groundwater. Aerobic biodegradation of sulfolane by Pseudomonas can be significantly enhanced through inoculation with sulfolane‐contaminated sediments or acclimatization to sulfolane environment. © 2022 Society of Chemical Industry