Abstract
The recombinant antibodies AE804, AE806, AE999, AF017, AF041 and AF131 detect by immunohistochemistry the insulin-secreting beta cells in mouse pancreatic islets. The most efficient staining was obtained with AE999.
Highlights
Pancreatic islet cells are highly differentiated and can be characterized by the distinctive hormones they produce: insulin for beta cells, glucagon for alpha cells, somatostatin for delta cells and pancreatic polypeptide for PP cells (Baskin, 2015)
We describe the ability of six recombinants antibodies (AE804, AE806, AE999, AF017, AF041 and AF131) to detect pancreatic insulinsecreting beta-cells by immunohistochemistry
The synthesized scFv sequences (GeneArt, Invitrogen) for AE804, AE806, AE999, AF017, AF041 and AF131 correspond to the sequence of the variable regions of the CG7C7
Summary
Pancreatic islet cells are highly differentiated and can be characterized by the distinctive hormones they produce: insulin for beta cells, glucagon for alpha cells, somatostatin for delta cells and pancreatic polypeptide for PP cells (Baskin, 2015). Antibodies: ABCD_AE804, ABCD_AE806, ABCD_AE999, ABCD_AF017, ABCD_AF041 and ABCD_AF131 antibodies (ABCD nomenclature, web.expasy.org/abcd/; Lima et al, 2020) were produced by the Geneva Antibody Facility Antigen: AE804, AE806, AE999 and AF131 were originally raised against human insulin (Uniprot #P01308) in mice (Ewulonu et al, 1990; Lake et al, 1994; Oyu and Thomas, 1997); AF017 and AF041 were originally identified as autoantibodies from either human patients with diabetes mellitus (Ikematsu et al, 1994) or from diabetic mice (Uniprot #P01326; Pléau et al, 1993) respectively.
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