Abstract
Abstract Callus culture establishment and adventitious shoot development from callus of Rhododendron × ‘Gibraltar’ (G) and R. × ‘Old Gold’ (OG) after serial subculture were determined. Callus grew on Anderson's Rhododendron medium containing 2,4- D at 0.0045, 0.009, or 0.018 mm, but was optimum at 0.018 mm, since shoot organogenesis was suppressed at that level. After two callus subcultures, adventitious shoot development from callus on media containing 0, 0.017, 0.034, 0.068, or 0.136 mm zeatin (mixed isomers) was optimum at 0.034 and 0.068 mm zeatin for G and OG, respectively. After 17 weeks (five subcultures), 70- to 75-mg pieces of G and OG callus regenerated ≈20 shoots each. Adventitious shoot regeneration from callus declined with prolonged callus subculture. Regenerated plants are currently being evaluated for somaclonal variation. Chemical names used: (2,4-dichlorophenoxy)acetic acid (2,4-D); (E)-2-methyl-4-(1H-purin-6-ylamino)-2-buren-1-ol (zeatin).
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