Abstract
Adventitious shoot induction was studied using isolated parts of in vitro grown seedlings of Eastern redbud. The explants studied included the cotyledon node (with or without cotyledons), cotyledon, hypocotyl, epicotyl, root, nodal stem and seedling leaf pieces. Explants were treated with a factorial combination of benzylaminopurine (BAP) and thidiazuron (TDZ) on DKW medium. Cultures of Eastern redbud produced a low frequency of adventitious shoot formation for all explants tested except for explants with the cotyledonary node. These explants produced 5.9 shoots per culture after treatment with 10μM BAP + 1μM TDZ. However, shoots produced on a medium containing TDZ failed to elongate. Many of the TDZ induced shoots were abnormal and distorted. Shoot elongation was promoted by transferring explants to a medium with BAP alone.
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