ADVENTITIOUS ROOT DEVELOPMENT IN TYPHA GLAUCA, WITH EMPHASIS ON THE CORTEX

Abstract

Adventitious root development was investigated in Typha glauca plants grown under experimental conditions with the previous year's dead, sterile stalk either emerged above or submerged below the surface of Hoagland's solution. Adventitious roots emerged from buds in which most primordia had been earlier formed. Most roots elongated to 14–19 cm in 3–4 weeks and produced abundant lateral roots to their tips. Root apical meristem organization was typically monocotyledonous with a single tier of ground meristem/protoderm over the procambium. The ground meristem had zones of periclinal divisions in its innermost and outermost layers; the innermost layer initiated the endodermis and midcortex, and the outermost layers initiated the hypodermis. Crystalliferous cells with raphides were produced in the midcortex, and aerenchyma resulted from the radial expansion of schizogenous air spaces and some lysigeny in the midcortex. The procambium produced a vascular cylinder with 10–13 phloem and xylem poles, 6–9 large metaxylem elements, and central sclerenchyma. As roots stopped elongating, they narrowed, the vascular cylinder diminished in size, typical aerenchyma was lost from the cortex, crystal production ceased, and the rootcap diminished in size with its storage starch used up. Growth was determinate in these adventitious roots. The results suggested that a periclinally derived outer ground meristem was a prerequisite for a hypodermis, which, in turn, was necessary as a structural framework for aerenchyma. Without a hypodermis, typical aerenchyma was not present.