Abstract
Primary leaves of garland chrysanthemum seedlings were cultured on MS medium supplemented with either 0.1 mg·liter-1 BA+0.1 mg·liter-1 NAA or 0.1 mg·liter-1 BA+1 mg·liter-1 NAA. Adventitious buds were mainly formed in the presence of 0.1 mg·liter-1 BA+0.1 mg·liter-1 NAA at the cut end of the explants 6-9 days after culture, whereas embryoids were mainly formed in the presence of 0.1 mg·liter-1 BA+1 mg·liter-1 NAA at the cut ends or in various areas of the explants 9-12 days after culture. The embryoids had cotyledon-and hypocotyl-like tissues, but lacked definite root structures. Histological observations revealed that these adventitious buds and embryoids were formed directly from meristematic tissues derived from epidermal and subepidermal tissues and did not originate from callus.
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