Abstract
Background The introduction of animal origin free (AOF) media has significantly reduced the incidence of adventitious virus contamination in biological production systems. Nevertheless, contamination by the parvovirus Mouse Minute Virus (MMV) remains a continuing challenge. Although infrequent, infection of a fermenter can be catastrophic for a manufacturer, and can also have a potential impact on drug supply, patient safety and have regulatory implications. In this work, we evaluated engineering Chinese Hamster Ovary (CHO) cell lines to create a new host cell line that
Highlights
The introduction of animal origin free (AOF) media has significantly reduced the incidence of adventitious virus contamination in biological production systems
We evaluated engineering Chinese Hamster Ovary (CHO) cell lines to create a new host cell line that
CHO cell lines engineered to express lower surface sialic acid demonstrate increased resistance to Mouse Minute Virus (MMV) infection compared to the wild-type controls
Summary
Adventitious agent contamination risk mitigation: engineering MMV virus resistance into CHO cells. Joaquina X Mascarenhas1*, Lisa Burger, Ademola Kassim, Trissa Borgschulte, Delia Lyons, Henry George, Nan Lin, Audrey Chang, David Onions, David Pintel, Kevin. From 24th European Society for Animal Cell Technology (ESACT) Meeting: C2P2: Cells, Culture, Patients, Products Barcelona, Spain. From 24th European Society for Animal Cell Technology (ESACT) Meeting: C2P2: Cells, Culture, Patients, Products Barcelona, Spain. 31 May - 3 June 2015
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