Abstract
Background: Biopsy of single cells from developing embryos is one of the steps of the Preimplantation Genetic Diagnosis (PGD) procedure. Chemical opening by acidified Tyrode’s solution and mechanical zona dissection (PZD) can lack precision and reproducibility because of the different skills among embryologists. The use of a laser in PGD provides a more precise and controlled opening from embryo to embryo and between operators.
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