Abstract
Processing of lignocellulosic biomass includes four major unit operations: pre-treatment, hydrolysis, fermentation and product purification prior to biofuel generation via anaerobic digestion. The microorganisms involved in the fermentation metabolize only simple molecules, i.e., monosugars which can be obtained by carrying out the degradation of complex polymers, the main component of lignocellulosic biomass. The object of this paper was to evaluate the saccharification conditions and identify the process parameters that should be applied to improve the saccharification efficiency of lignocellulosic biomass, defined as the simple sugars concentration, which was considered as a crucial parameter for hydrogen generation via dark fermentation. Drawing global conclusions about the occurring changes in the biomass requires learning about the nature of the biomass structure and composition at different stages of the process. Therefore, techniques for analysis, as FTIR, HPLC and SEM were applied. The experiment was planned employing Box–Behnken design. The advantageous operating conditions and the composition of saccharification enzymatic cocktail were identified and their values occurred similar in the applied border conditions for all tested biomass types. Analysis of the intermediate solid and liquid streams generated during the pre-treatment procedure revealed several structural and compositional changes in the biomass.
Highlights
The trend to produce second-generation biofuels from nonconsumer raw materials increases
Biomass residues contain lignocellulose that is composed mainly of cellulose, This work was presented at the 44th International Conference of Slovak Society of Chemical Engineering held in Tatranské Matliare on May 21–25, 2018
The object of this paper is to evaluate the advantageous saccharification conditions and identify the process parameters that should be applied to improve the saccharification efficiency of lignocellulosic biomass, defined as the simple sugars concentration, which is considered as a crucial parameter for hydrogen generation via dark fermentation
Summary
The trend to produce second-generation biofuels from nonconsumer raw materials increases. The recovery of energy and the reduction of the content of organic waste are the benefits of the lignocellulosic residue application within the bioconversion processes. Biomass residues contain lignocellulose that is composed mainly of cellulose (about 50% wt.), This work was presented at the 44th International Conference of Slovak Society of Chemical Engineering held in Tatranské Matliare on May 21–25, 2018. The processing of lignocellulosic biomass to hydrogen includes four major unit operations: pre-treatment, hydrolysis, fermentation and product purification. Pre-treatment includes milling, mincing and biomass conditioning; it is a high-cost consuming process. Size reduction of biomass particles is used for most lignocellulosic raw materials and it is realized by means of fragmentation, grinding, milling or defibering. The hydrolysis is carried by means of chemical,
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