ADVANCING PARKINSON’S DISEASE DETECTION WITH FLISA: A NOVEL APPROACH TARGETING MISFOLDED ALPHA-SYNUCLEIN

Paramita Batabyal,Dbt-Ict Centre For Energy Biosciences, Institute Of Chemical Technology, Mumbai - 400 019, Maharashtra, India ,Vishwas Sarangdhar,Ajay S. Sawner,Amruta V. Gadade,Radni D. Deshpande,Vikas D. Dighe,Sadhana Sathaye,Caius Research Laboratory, St. Xavier’s College, Mumbai – 400 001, Maharashtra, India ,Biological Sciences And Bioengineering, Indian Institute Of Technology, Mumbai – 400 076, Maharashtra, India ,Icmr -National Centre For Preclinical Reproductive And Genetic Toxicology, Mumbai – 400 012, Maharashtra, India ,Department Of Pharmaceutical Sciences And Technology, Institute Of Chemical Technology, Mumbai – 400 019, Maharashtra, India ,Icmr -National Centre For Preclinical Reproductive And Genetic Toxicology, Mumbai – 400 012, Maharashtra, India ,Department Of Pharmaceutical Sciences And Technology, Institute Of Chemical Technology, Mumbai – 400 019, Maharashtra, India

doi:10.53879/id.61.03.14385

Paramita Batabyal, Dbt-Ict Centre For Energy Biosciences, Institute Of Chemical Technology, Mumbai - 400 019, Maharashtra, India + Show 12 more

https://doi.org/10.53879/id.61.03.14385

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Journal: INDIAN DRUGS

Publication Date: Mar 28, 2024

Abstract

A Fluorescence-Linked Immunosorbent Assay (FLISA) targeting β-cross-sheet α-synuclein protein, a crucial marker in Parkinson’s Disease (PD), was assessed using in vitro assay with a rotenone-induced PD cellular model. The study aimed to evaluate FLISA’s applicability with the inhouse developed labelled polyclonal antibody (pAb). Results are promising the competitive assay successfully distinguished misfolded α-synuclein in SH-SY5Y cells. Quantitative analysis revealed a direct correlation between competitive antigen concentration and decreased fluorescence, showcasing FLISA’s sensitivity. Controls exhibited maximal fluorescence, confirming the absence of misfolded proteins, while rotenone exposed cells displayed reduced fluorescence, suggesting their presence. This approach enhances PD understanding and supports potential interventions.

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