Abstract
The measurement of volatile organic compounds (VOCs) originating from organisms allows continuous monitoring and a unique insight into the metabolism. One method offering the sensitivity to detect these VOCs is secondary electrospray ionization coupled to high-resolution mass spectrometry (SESI-HRMS). SESI was derived from electrospray ionization (ESI) and has found widespread application in clinical research and monitoring of animals. This review discusses the technical aspects behind SESI, the advancements, and the technical hurdles faced. Additionally, the recent advances in the applications of SESI in human and animal-centered research are presented.
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