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Abstract
Abstract Microdroplets have been widely used in biology, chemistry, material science and engineering, and the microdroplet generation methods are becoming more efficient and controllable. The development of the generation of microdroplets experienced tradition methods, microfluidic chip-based methods, and off-chip methods proposed recently. In this review, we first briefly summarized the typical traditional methods and the microfluidic chip-based methods for microdroplet generation, and then emphatically introduced the principles, devices, and applications of the recently developed microdroplet generation methods. The advantages and disadvantages of these methods were discussed and the future development of microdroplet generation technology was also prospected.
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