Abstract
This chapter discusses the use of hydrophilic interaction chromatography (HILIC) for analysis of biochemical compounds. Some compounds such as amino acids, carbohydrates, nucleobases, nucleosides, oligonucleotides, peptides, phospholipids, and proteins are of interest as components in pharmaceutical or food products. The chapter focuses on application of HILIC to separations of these types of compounds in problems of biochemical and related interests. Many recent glycan analysis applications have employed zwitterionic- HILIC (ZIC-HILIC) or neutral amide stationary phases. Other types of HILIC columns have been used for certain applications, but it seems that the field is moving toward the former two column types as first-line choices. Fluorescence detection of derivatized glycans or mass spectrometric detection is clearly the mode of choice, with mass spectrometry (MS) providing superior data for structural characterization. Amide or zwitterionic phases have also become popular for other biomolecules such as nucleobases, nucleosides, nucleotides, oligonucleotides, amino acids, and peptides.
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