Abstract
Advances in detection methods for Shiga toxin-producing Escherichia coli (STEC).
Highlights
Shiga toxin-producing Escherichia coli (STEC) comprise E. coli strains capable of producing toxins named Shiga toxin type 1 (Stx1), type 2 (Stx2), or both, encoded by stx1 and stx2 genes, respectively
Both STEC O157 and non-O157 STEC have been detected using molecular methods that do not exert selection pressure toward any particular serotype, such as detecting stx in samples by PCR followed by isolation of colonies, detection of stx1, stx2, eae, saa, ehxA by multiplex PCR and serotyping (Scheutz et al, 2001; Fernández et al, 2010; Piazza et al, 2010) but this procedure is laborious and time consuming
Commercial test kits for screening from enrichment cultures based on immunological and molecular methods have been developed to detect stx, eae, and serogroups frequently associated with severe human disease (O157, O26, O103, O111, and O145)
Summary
Shiga toxin-producing Escherichia coli (STEC) comprise E. coli strains capable of producing toxins named Shiga toxin type 1 (Stx1), type 2 (Stx2), or both, encoded by stx1 and stx2 genes, respectively. A commentary on Detection of Shiga toxin-producing Escherichia coli in ground beef using Genedisc real-time PCR system by Fratamico, P. Since 1982 when STEC O157:H7 was first reported, the selective diagnostic methods have used particular features of this serotype to increase its isolation.
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