Abstract
Abstract High-speed countercurrent chromatography (HSCCC) is a novel, efficient, and productive chromatographic technique based on continuous liquid-liquid partition, which has the unique feature of eliminating the irreversible adsorption using liquid support, and is widely used in the fields of traditional Chinese medicine, biochemistry, food, natural product chemistry, environment analysis, and so on. In this study, the application of HSCCC in the separation and purification of known and unknown flavonoids is reviewed. Furthermore, this study introduces the developments in HSCCC techniques and their application in the separation and purification of flavonoids and other compounds.
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