Abstract
Aflatoxin (AF) contamination is a major concern in the food and feed industry because of its prevalence and toxicity. Improved aflatoxin detection methods are still needed. Immunoassays are an important method for total aflatoxin (TAF) analysis in food due to its technical advantages such as high specificity, sensitivity, and simplicity, but require high-quality antibodies. Here, we first review the three ways to prepare high-quality antibodies for TAF immunoassay, second, compare the advantages and disadvantages of antigen synthesis methods for B-group and G-group aflatoxins, and third, describe the status of novel genetic engineering antibodies. This review can provide new methods and ideas for the development of TAF immunoassays.
Highlights
Aflatoxins (AFs) are a group of toxic secondary metabolites containing similar molecular structures
The 2-carbonyl group of Aflatoxin B1 (AFB1) was selected as the active site, the active group carboxyl was introduced by oximation, and the monoamide bond was used as the spacer arm to synthesize the antigen
The 3 and 4-position furan ring of AFB1 was selected as the active site, AFB1 epoxides were formed through oxidation, hydroxyl groups were introduced, carboxyl groups were introduced in reaction with anhydride, and monoamide bond was used as the spacer arm to synthesize antigen
Summary
Aflatoxins (AFs) are a group of toxic secondary metabolites containing similar molecular structures (difuran ring and oxyheteronaphthalidone). The preparation of high-quality antibodies is in the screening of TAF due to their high selectivity, strong sensitivity, rapid and simple sample a key technology to establish immunoassay methods. According to the current research progress, there are three ways a single monoclonal antibody (mAb) of B-group AFs and G-group AFs with high sensitivity and to prepare high-quality antibodies for TAF immunoassay: The first is to prepare a single monoclonal strong specificity. These are mixed with the universal antibody.
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