Abstract
Soybean is one of humanity's major sources of plant protein. It is also very important for animal feed and as industrial raw material. Great advances have recently been achieved in its genetic transformation. This review provides a comprehensive discussion of important factors affecting Agrobacterium-mediated soybean transformation including target tissues, plant tissue health, wounding methods, regeneration systems, selectable markers and reporter genes.
Highlights
Soybean [Glycine max (L.)Merrill.] has been widely used as human and animal food, as well as an industrial raw material
This review provides a comprehensive discussion of important factors affecting Agrobacterium-mediated soybean transformation including target tissues, plant tissue health, wounding methods, regeneration systems, selectable markers and reporter genes
Transgenic soybean plants have been produced by Agrobacterium-meditated T-DNA delivery into cotyledonary nodes (Hinchee et al, 1988), immature cotyledons (Parrott et al, 1994), and embryogenic suspension cultures (Trick & Finer, 1998)
Summary
Soybean [Glycine max (L.)Merrill.] has been widely used as human and animal food, as well as an industrial raw material. Transgenic soybean plants have been produced by Agrobacterium-meditated T-DNA delivery into cotyledonary nodes (Hinchee et al, 1988), immature cotyledons (Parrott et al, 1994), and embryogenic suspension cultures (Trick & Finer, 1998). Particle bombardment–meditated transformation is more commonly used to achieve transformation of many soybean tissues including shoot meristems (McCabe et al, 1988; Aragão et al, 2000) and embryogenic suspension cultures (Finer & McMullen, 1991) without the host-range limitation associated with Agrobacterium This approach, tends to result in the integration of large complexes or fragments of transgenes that sometimes leads to gene silencing (Vaucheret et al, 1998; Dai et al, 2001). Cotyledons were inoculated with Agrobacterium and placed on an embryo induction medium followed by transfer to a regeneration medium This method differs from de cot node system in that plants were regenerated from somatic embryos rather than from shoots. The half-seed system was reported as simple and does not require deliberate wounding of explants, which is a critical and technically demanding step in the cotyledonary node method (Paz et al, 2005)
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