Abstract
Corynebacterium glutamicum has been considered a promising synthetic biological platform for biomanufacturing and bioremediation. However, there are still some challenges in genetic manipulation of C. glutamicum. Recently, more and more genetic parts or elements (replicons, promoters, reporter genes, and selectable markers) have been mined, characterized, and applied. In addition, continuous improvement of classic molecular genetic manipulation techniques, such as allelic exchange via single/double-crossover, nuclease-mediated site-specific recombination, RecT-mediated single-chain recombination, actinophages integrase-mediated integration, and transposition mutation, has accelerated the molecular study of C. glutamicum. More importantly, emerging gene editing tools based on the CRISPR/Cas system is revolutionarily rewriting the pattern of genetic manipulation technology development for C. glutamicum, which made gene reprogramming, such as insertion, deletion, replacement, and point mutation, much more efficient and simpler. This review summarized the recent progress in molecular genetic manipulation technology development of C. glutamicum and discussed the bottlenecks and perspectives for future research of C. glutamicum as a distinctive microbial chassis.
Highlights
Corynebacterium glutamicum has been widely used in the food industry for amino acid production (Wendisch et al, 2016)
The genetic modification of C. glutamicum can be traced back to 1984 (Ozaki et al, 1984), but the development and application of genetic manipulation technology are progressing slowly (Nesvera and Patek, 2011; Suzuki and Inui, 2013; Yang et al, 2020), which may be attributed to the fact that C. glutamicum is a type of Gram-positive actinomyces with high GC content in the genome (Ikeda and Nakagawa, 2003)
More and more genetic manipulation tools have been applied in C. glutamicum (Nesvera and Patek, 2011; Suzuki and Inui, 2013), including type strain ATCC 13032 and no-model industrial strains such as Brevibacterium flavum and Corynebacterium crenatum (Xu et al, 2010; Shu et al, 2018)
Summary
Corynebacterium glutamicum has been considered a promising synthetic biological platform for biomanufacturing and bioremediation. There are still some challenges in genetic manipulation of C. glutamicum. Continuous improvement of classic molecular genetic manipulation techniques, such as allelic exchange via single/doublecrossover, nuclease-mediated site-specific recombination, RecT-mediated singlechain recombination, actinophages integrase-mediated integration, and transposition mutation, has accelerated the molecular study of C. glutamicum. Emerging gene editing tools based on the CRISPR/Cas system is revolutionarily rewriting the pattern of genetic manipulation technology development for C. glutamicum, which made gene reprogramming, such as insertion, deletion, replacement, and point mutation, much more efficient and simpler. This review summarized the recent progress in molecular genetic manipulation technology development of C. glutamicum and discussed the bottlenecks and perspectives for future research of C. glutamicum as a distinctive microbial chassis
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
