Abstract

With the development of bioinspired green solutions for sustainable construction over the past two decades, bio-cementation, which exploits the naturally occurring phenomenon of calcium carbonate precipitation in different environments, has drawn a lot of attention in both building construction and soil stabilization. Various types of microorganisms, along with specific enzymes derived from these microorganisms, have been utilized to harness the benefits of bio-cementation. Different application methods for incorporating this mechanism into the production process of the construction material, as well as a variety of experimental techniques for characterizing the outcomes of bio-cementation, have been developed and tested. Despite the fact that the success of bio-cementation as a sustainable method for construction has been demonstrated in a significant body of scientific literature at the laboratory scale, the expansion of this strategy to construction sites and field application remains a pending subject. The issue may be attributed to two primary challenges. Firstly, the complexity of the bio-cementation phenomenon is influenced by a variety of factors. Secondly, the extensive body of scientific literature examines various types of microorganisms under different conditions, leading to a wide range of outcomes. Hence, this study aims to examine the recent advancements in utilizing the most commonly employed microorganism, Sporosarcina pasteurii, to emphasize the significance of influential factors identified in the literature, discuss the findings that have been brought to light, and outline future research directions toward scaling up the process.

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