Advanced synthesis and application of Nano SiC@ β-glucosidase@ Fe3O4 composite

Abstract

An improved method for immobilizing β-glucosidase was studied in present work. The immobilization included the following steps: preparing a nano silicon carbi de carrier, mixing the carrier, β-glucosidase and a cross-linking agent to obtain immob ilized β-glucosidase. Then, the immobilized β-glucosidase was mixed with Fe3O4 nano particles and self-assembled to obtain nano-silicon carbide@β-glucosidase@ Fe3O4 co mposite. This method improved the activity of immobilized β-glucosidase, and solved the technical problems of low β-glucosidase reuse rate and low enzyme activity retenti on rate.