Abstract
Industrial enzymes, such as glucoamylase and amylase etc., are important biocatalysts which are widely used in many fields. In this paper, advanced strategies for improving the production of industrial enzymes in heterologous host systems were reviewed from the following aspects: the introduction of the strong promoter, the increase in the copy number of genes, the alternative to the signal peptide and the use of preferred codons.
Highlights
Traditional monospore isolation, mutation breeding and optimization of fermentation process have been widely used to improve the production of industrial enzymes
In this paper, advanced strategies for improving the production of industrial enzymes were reviewed from the following aspects: the introduction of the strong promoter, increasing the copy number of genes, changing the signal peptide sequence and designing the codon bias (Figure 1)
Use of the strong promoter to improve the production of industrial enzymes Introducing exogenous genes to host strains often results in strains that cannot start the expression of exogenous genes
Summary
Traditional monospore isolation, mutation breeding and optimization of fermentation process have been widely used to improve the production of industrial enzymes. Genes of some enzymes, such as glucoamylase, amylase, lipase, cellulase and phytase, etc., have been cloned and expressed efficiently in heterologous host systems, such as Saccharomyces cerevisiae; Aspergillus niger and Pichia pastoris [111].
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