Abstract
Myocardial infarction represents the most investigated pathology. Heart tissues are histologically assayed on a routine base in clinical laboratories. However, the lack of a standard operation procedure for e.g. calculating the size of the infarcted area of myocardium, may lead to an increased errors’ interval, with little correlation between results of a same tissue and/or with other pathophysiological parameters. This creates a clear barrier for further development of novel therapeutic strategies. In the present study, we present the robust applicability of a novel methodology such as: advanced modular automated calculation of (i) the size of infarcted heart of mice, (ii) the net collagen content present in the scar, and (iii) the interstitial fibrosis in remote, which are simultaneously performed. The new approach of defining the infarct size, one of the important predictor of every cardiac therapeutic intervention, will create a positive impact in the research accuracy. Additionally, it will be expected that the readily assembled reports of simultaneously computed parameters and its user-friendly operation allow an efficient and effective estimation of measurements.
Highlights
Myocardial infarction represents the most common cause of death in the world
We present the robust applicability of a novel methodology such as: advanced modular automated calculation of (i) the size of infarcted heart of mice, (ii) the net collagen content present in the scar, and (iii) the interstitial fibrosis in remote, which are simultaneously performed
The localization and estimation of the infarcted size is made by using dedicated software[3]. This method is applied for large animals in addition to functional studies such as: the assessment of serum markers, technetium-99m sestamibi single-photon emission computed tomography (SPECT) myocardial perfusion imaging or magnetic resonance imaging[4]
Summary
Myocardial infarction represents the most common cause of death in the world. huge efforts are made to investigate the patho-physiological mechanisms of the initiation, progression, and healing of heart after the acute myocardial infarction[1]. The lack of a standard operation procedure for, e.g. calculating the size of the infarcted area of myocardium, may lead to an increased errors’ interval, with little correlation between results of a same tissue and/or with other pathophysiological parameters. This creates a clear barrier for further development of novel therapeutic strategies. The localization and estimation of the infarcted size is made by using dedicated software[3] This method is applied for large animals (rats, rabbits) in addition to functional studies such as: the assessment of serum markers, technetium-99m sestamibi single-photon emission computed tomography (SPECT) myocardial perfusion imaging or magnetic resonance imaging[4]. The latter will become more expensive to be applied in small animal models
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
