Abstract
By eliminating the photodamage and photobleaching induced by high intensity laser and fluorescent molecular, the label-free laser scanning microscopy shows powerful capability for imaging and dynamic tracing to biological tissues and cells. In this review, three types of label-free laser scanning microscopies: laser scanning coherent Raman scattering microscopy, second harmonic generation microscopy and scanning localized surface plasmon microscopy are discussed with their fundamentals, features and recent progress. The applications of label-free biological imaging of these laser scanning microscopies are also introduced. Finally, the performance of the microscopies is compared and the limitation and perspectives are summarized.
Highlights
To date, optical microscopy has been the most important tool in biology for observing the biological structures, morphology and dynamics in subcellular size
Various super resolution microscopies have been developed based on fluorescent confocal laser scanning microscopy (FCLSM), such as stimulated emission depletion (STED) [2,3,4], reversible saturable optical fluorescence transitions (RESOLFT) [5,6,7], and saturated excitation (SAX) [8,9]
Three types of label-free laser scanning microscopy are mentioned with their particular features
Summary
Optical microscopy has been the most important tool in biology for observing the biological structures, morphology and dynamics in subcellular size. Advanced Label-Free Laser Scanning Microscopy and Its Biological Imaging Application. The fluorescent confocal laser scanning microscopy (FCLSM) that combines the fluorescent labels and CLSM has been one of the most powerful and versatile methods for studying cells, tissues and organisms.
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