Advanced interfere prevention of diabetic cardiomyopathy rats by acid fibroblast growth factor-loaded heparin-modified nano-liposomes and ultrasound-targeted microbubble destruction technique

Abstract

Objective To investigate the advanced preventive effect of acid fibroblast growth factor (aFGF) on diabetic cardiomyopathy (DCM) by using heparin-modified nano-liposomes combined with ultrasound-targeted microbubble destruction technique (UTMD) . Methods aFGF-loaded nano-liposomes (aFGF-lips) were prepared by lyophilization technique. Type Ⅰ diabetes model was induced by intraperitoneal injection of streptozotocin (STZ, 70 mg/kg) in male SD rats. Before and twelve weeks after intervention, all rats underwent the transthoracic echocardiography. The segmental mean peak systolic radial velocity (Vs) , systolic circumferential strain (Sc) , and systolic circumferential strain rate (SRc) were measured. The expression of aFGF in DCM rats was detected by western blot. The rats were sacrificed and myocardial tissue were stained with masson staining and Tunel staining to quantify myocardial collagen fraction (CVF) and cardiac myocyte apoptosis index (AI) . Results aFGF-lips showed good morphology and aFGF encapsulation efficiency (89.4±1.2) % with high stability. From the animal experiments, the echocardiographic indexes including Vs, Sc and SRc had significantly improvements over DM group (P<0.05) and all other treatment group (P<0.05) . The Masson’s trichrome staining demonstrated that CVF was significantly higher in DM group than in the control group and was significantly lower in the aFGF-loaded nano-liposome+UTMD group than other groups (all P<0.05) . The TUNEL results showed that AI was significantly higher in DM group than in the control group and was significantly lower in aFGF-loaded nano-liposome+UTMD group than other groups (all P<0.05) . Conclusion aFGF nano-liposome combining with UTMD technique can improve the functions and pathologies of the hearts in type 1 diabetes mellitus model, which might provide a novel technique for aFGF in DCM prevention. Key words: Diabetic cardiomyopathy; Acid fibroblast growth factor; Ultrasound-targeted microbubble destruction technique; Heparin-modified nano-liposomes