ADVANCED INDUSTRIAL FIRST AID, 1947

Abstract

<h3>Background</h3> Sexual transmitted infections are playing an important role in genital infections. <i>Chlamydia trachomatis</i> (CT), <i>Neisseria gonorrhoeae</i> (NG), <i>Mycoplasma genitalium</i> (MG) and <i>Trichomonas vaginalis</i> (TV), have been associated to vaginal infections, cervicitis and urethritis tand complications like pelvic inflammatory diseases. Pathogens like <i>Mycoplasma hominis</i> (MH), <i>Ureaplasma urealyticum</i> (UU) and <i>Ureaplasma parvum</i> (UP), residing in the genital tracts, are not always associated to active infections. Seegene introduced the Allplex™ CT/NG/MG/TV (STI-4) Assay. This study objective was to compare the performance of the Anyplex™II STI-7e v1.1 (STI-7) that detects CT, NG, TV), MG, MH, UU), and UP, to the Allplex™ CT/NG/MG/TV for the detection of STI from urines. <h3>Methods</h3> Urines collected with UriSponge™ (Copan Italia, Brescia Italy) were tested with the STI-7: UriSpone^TM samples were centrifuged 5min at 2500 RPM, tubes de-capped, sponges discarded, vortexed and loaded samples on the Nimbus for nucleic acids extraction. Extracted samples were analyzed with the STI-7 on the CFX96 system. Nucleic acids from 142 urine, already defined negative and positive, were analyzed with the Allplex™ CT/NG/MG/TV on the CFX96 system. Both assays were were compared, concordant results were considered negative or positive, discordant results were retested in duplicate. <h3>Results</h3> In the 142 urines tested with STI-7 and STI-4 assays, concordant results were detected, 29 CT, 16 NG, 11 MG positives, 29 negatives and 5 discordant results, 2 STI-7 negative/STI4 positive (1MG and 1CT) and 3 STI-7 positive/STI-4 negative (1NG, 1CT,1TV). Results were not statistically significant (P &gt;0.05). Anyplex II STI-7 detected 22 MH, 25 UU and 45 UP. <h3>Conclusion</h3> In this study an excellent agreement was demonstrated by the Seegene Anyplex™II STI-7e v1.1 and by Allplex™ CT/NG/MG/TV assays for the detection of <i>Chlamydia trachomatis</i>, <i>Neisseria gonorrhoeae, Mycoplasma genitalium</i> (MG) from urine collected with Copan UriSponge^TM. The Allplex™ CT/NG/MG/TV assays eliminate the detection of MH, UU, and UP. <h3>Disclosure</h3> No significant relationships.