Abstract
Live cell imaging is now used across all domains of cell and developmental biology (1). Combined with the straightforward facility for molecular labeling via fluorescent proteins and other labeling techniques, live cell imaging gives unparalleled access to the dynamics of ions and molecules in living cells and the patterns of growth and movement of cells in living tissues. Many critical insights into the biology of the cells of microorganisms, plants, and animals have been revealed through live cell imaging, and its applications only grow with every new labeling tool or imaging modality that is reported. Despite this success, live cell imaging is technically demanding and is a technique that has substantial limitations. Microscopy has recently enjoyed great success in breaking well-known optical limits in spatial resolution (2, 3), but for studies of dynamics in live cells, temporal resolution is at least as important—temporal aliasing causes significant artifacts and can hide biologically important phenotypes and mechanisms (4).
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