Year-end Sale % OFF 
Abstract
The molecular genetic basis of cotton fiber strength and fineness in crosses between Gossypium mustelinum and Gossypium hirsutum (Upland cotton) was dissected using 21 BC3F2 and 12 corresponding BC3F2:3 and BC3F2:4 families. The BC3F2 families were genotyped with simple sequence repeat markers from a G. hirsutum by G. mustelinum linkage map, and the three generations of BC3-derived families were phenotyped for fiber strength (STR) and fineness (Micronaire, MIC). A total of 42 quantitative trait loci (QTLs) were identified through one-way analysis of variance, including 15 QTLs for STR and 27 for MIC, with the percentage of variance explained by individual loci averaging 13.86 and 14.06%, respectively. Eighteen of the 42 QTLs were detected at least twice near the same markers in different generations/families or near linked markers in the same family, and 28 of the 42 QTLs were identified in both mixed model-based composite interval mapping and one-way variance analyses. Alleles from G. mustelinum increased STR for eight of 15 and reduced MIC for 15 of 27 QTLs. Significant among-family genotypic effects (P < 0.001) were detected in 13 and 10 loci for STR and MIC respectively, and five loci showed significant (P < 0.001) genotype × family interaction for MIC. These results support the hypothesis that fiber quality improvement for Upland cotton could be realized by introgressing G. mustelinum alleles although complexities due to the different effects of genetic background on introgressed chromatin might be faced. Building on prior work with G. barbadense, G. tomentosum, and G. darwinii, QTL mapping involving introgression of G. mustelinum alleles offers new allelic variation to Upland cotton germplasm.
Highlights
This is the fourth report describing the interspecific G. mustelinum by G. hirsutum (Upland cotton) genetic map and mapping and introgressing quantitative trait loci (QTLs) of fiber quality traits from G. mustelinum into Upland cotton
Three generations of interspecific advanced- backcross populations, namely 21 BC3F2 families and 12 BC3F2:3 and BC3F2:4 families were developed as follows: a G. hirsutum acc., PD94042 was crossed with G. mustelinum (AD4-8), and F1 plants were independently backcrossed to the G. hirsutum parent for three cycles; a total of 21 lineages that produced BC3F1 plants were self-pollinated and generated 21 BC3F2 families with size ranging from 127 to 160 plants; in addition, 12 BC3F2:3 and BC3F2:4 families with size ranging from 130 to 160 lines were developed for 12 of the 21 BC3F2 families with enough seeds (Table S1)
The 21 BC3F2 families were planted in 2006; completely randomized designs were used for the 12 BC3F2:3 and BC3F2:4 families with two replicate plots in two years (2008 and 2009) in Tifton, Georgia
Summary
This is the fourth report describing the interspecific G. mustelinum by G. hirsutum (Upland cotton) genetic map and mapping and introgressing quantitative trait loci (QTLs) of fiber quality traits from G. mustelinum into Upland cotton. We reported the interspecific G. hirsutum by G. mustelinum genetic linkage map (Wang et al, 2016b), and described 24 QTLs. QTL Mapping of Fiber Strength and Fineness for fiber elongation (Wang et al, 2016a,b) and 65 QTLs for fiber length traits (Wang et al, 2017). Some alleles from G. mustelinum improved fiber properties, demonstrating their potential value for improving fiber quality in Upland cotton breeding. Interspecific QTL mapping and introgression were reported for two important fiber quality traits, fiber strength (STR) and fineness measured in Micronaire (MIC).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
