Advanced analytical tools for bovine lactoferrin identification and quantification in raw skim milk to finished lactoferrin powders

Abstract

As the use of bovine lactoferrin (bLF) has increased, greater regulatory and commercial interest is being placed on bLF identification and quantitation and quality. Several methods can be used to quantitate bLF, but a robust accurate quantification method applicable as a standardised tool for feed milk, process monitoring, and evaluation of end products has been less of a concern. bLF concentrations increase through the bovine lactation cycle, so accurate and timely information is required that can increase both processing plant performance and product quality and output. In this article we evaluate analytical cation exchange, reverse phase high-performance chromatography and size exclusion chromatography methods for the analysis of bLF identification and quantification from whole milk, process intermediates, ingredients, milk and infant formula products. However, the methods described in this article to identify and quantify the bLF may not be directly assimilated to the biological activities of the bLF.