Abstract
ObjectiveTo determine the intrinsic toxicity of hexane, ethyl acetate and methanol crude extracts of Ageratum houstonianum leaves against adult Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus mosquitoes. MethodsBioassay was performed in 2-day-old laboratory reared unfed adult female mosquitoes by topical application at concentrations of 0.01, 0.05, 0.10, 0.25 and 0.50 μg/mg female adult mosquito. ResultsAedes aegypti was found to be more susceptible to ethyl acetate and hexane extracts with LD50 value of 0.10 μg/mg, and both Anopheles stephensi and Culex quinquefasciatus were susceptible to methanol extract with LD50 values of 0.12 μg/mg female adult mosquito. ConclusionsThe results show promising adulticidal activity on topical application and further studies followed by in-depth laboratory and field bioassays are needed to screen, isolate and purify bioactive phytochemical constituents or compounds.
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