Abstract
Adult vial testing procedures were used to determine susceptibility of male corn earworm (Helicoverpa zea) to methomyl. Rate response bioassays using 0.39, 0.78, 1.56, 3.12, 6.25, 12.5, and 25 μg ai/vial were conducted using a laboratory colony. Results showed 10, 30, 70, 80, 80, 90, and 100% mortality at these rates. Field-collected moths from pheromone traps in Virginia were assayed at 0.25, 0.5, 1, 2, 5, and 10 times the 25 μg ai/vial rate (100% laboratory colony mortality rate). After 24 h exposure, 3.7 and 2.6% of moths survived the 12.5 and 25 μg ai/vial rates, with 100% mortality at all other rates. These results indicate that future assays should include lower rates in order to establish a baseline rate range for detecting susceptibility or resistance. Accepted for publication 18 December 2007. Published 12 March 2008.
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