Abstract
An electrochemical stripping procedure is described for trace measurements of adenine by adsorption on a static mercury drop electrode. Cyclic voltammetry was used to characterize the interfacial and redox behavior. Optimum experimental conditions were found when using a 0.001 M NaOH solution, initial potential of + 0.01 V, scan rate of 5 mV s−1 and linear scan mode. The response of free adenine is linear over the concentration range 0.25–1.25 ppm. Linear scan voltammetry, without accumulation yields a detection limit of 55 ppb adenine. Proper conditions were tested to measuring adenine in presence of guanine, acridine orange, copper, zinc and ssDNA.
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