Adsorptive endocytosis of proteins by eosinophil granulocytes in small intestine mucosa

Abstract

a non-fluorescent oil, and covered with a cover slip. For observation and measurement of fluorescence, an Olympus MMSP (multi-purpose microspectrophotometer) Type RF was used, combined with a rapid scanning recorder. A microbeam of UV light, 350 nm, 40 ~tm in diameter, was projected from above, perpendicular to one prism face of the zircon crystal and directed so as to avoid any inclusions or cracks. The fluorescence was examined through a barrier filter L-420. The fluorescence spectrum was recorded automatically between 450 and 700 nm. The light source was an Olympus ultra-high pressure mercury lamp, and the excitation filter was UG-1. There were rounded purple zircons, about 188x63 pm, and euhedral colorless zircons, about 146 x 42 gm in size, together in the same preparation, separated from the same specimen. The euhedral zircons were bipyramid crystals having combinations of (111) (100), (111) (110), or (111) (100) (110). All the zircons exhibited fluorescence mostly in a flesh color in various degrees. The spectra of rounded zircons are all alike, having a peak at 550 nm with symmetrical slopes on both sides (Fig. 1 A). Differing from this, those of euhedral zircons are divided into two types. Type 1 is characterized as having a peak at 550nm and a shoulder at 490nm (Fig. 1 B). Type 2 is that which has the major peak at 490 nm and another subsidiary peak at 580 nm (Fig. 1 C). Most of the euhedral zircons belong to the former type, and the latter is rare. Fluorescence of zircon has already been reported by some authors [3, 4]. However, the fluorescence spectrum of the individual zircon grain was not always clarified. According to the present research, as is seen in the above results, the type of fluorescence spectrum is different for rounded and euhedral zircons. Moreover, the spectra of the euhedral zircons are not always the same, in spite of the fact that their color and crystal form are indentical. Therefore, measurement of the fluorescence spectrum will be a new method for distinguishing morphologically identical but physically different zircons within the same sandstone. It will open a new way for investigating the hinterland of a sedimentary basin.