Adsorption of organic matter on titanium surfaces with nano- and micro-scale roughness studied with the electrochemical quartz crystal microbalance dissipation technique.

Claes-Olof A Olsson,Anna Neus Igual-Muñoz,Stefano Mischler

doi:10.1116/6.0001233

Claes-Olof A Olsson, Anna Neus Igual-Muñoz + Show 1 more

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https://doi.org/10.1116/6.0001233

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Journal: Biointerphases	Publication Date: Sep 1, 2021
Citations: 2	License type: cc-by

Affiliation: École Polytechnique Fédérale de Lausanne

Abstract

Adsorption of calf serum organic matter from a phosphate-buffered solution was studied using the electrochemical quartz crystal microbalance with additional dissipation measurements. Two types of crystal surfaces were used: one rough with micrometer-range surface features and one with roughness in the low nanometer range. The results showed that the adsorption of the organic material was about 1.5 orders of magnitude larger on the rough surface and almost independent of serum concentration in the electrolyte. The adsorption rates were found to increase with increasing serum concentration. For rough crystals, the adsorption kinetics were interpreted with the Johnson-Mehl-Avrami-Kolmogorov model, indicating an initial growth phase according to the tn-law, followed by a slower growth as the nucleation sites fill up. This study suggests that specific surface sites are critical to promote adsorption of proteins on a titanium surface.

Highlights

The interaction between a passive metal surface and corporal fluids is interesting from many aspects: improving the in-growth of fixed parts in a prosthesis, understanding wear phenomena in articulating parts in hip joints, or avoiding inflammatory reaction and possibly rejections of materials implanted in the body
The results showed that the adsorption of the organic material was about 1.5 orders of magnitude larger on the rough surface and almost independent of serum concentration in the electrolyte
The adsorption kinetics were interpreted with the Johnson–Mehl– Avrami–Kolmogorov model, indicating an initial growth phase according to the tn-law, followed by a slower growth as the nucleation sites fill up

Summary

Introduction

The interaction between a passive metal surface and corporal fluids is interesting from many aspects: improving the in-growth of fixed parts in a prosthesis, understanding wear phenomena in articulating parts in hip joints, or avoiding inflammatory reaction and possibly rejections of materials implanted in the body. One can find a number of ideas on how the proteins may interact with a metal surface, many of which start off with a Langmuir isotherm and add modifications to accommodate effects of rotating the protein, concentration, and serum composition. We investigate the properties of the metal surface with particular focus on how roughness influences protein adsorption. Protein interaction with metal surfaces is a highly complex phenomenon. It has been reviewed by Rabe et al. and more recently by Talha et al.. Rabe et al list the important parameters for protein adsorption, starting with pH. If it is close to the isoelectric point, the protein is neutral. Protein orientation effects can be used to explain overshoot phenomena during avs.scitation.org/journal/bip adsorption. The interaction between bovine serum albumin and biomedical alloys has been reviewed by Klok et al. For CoCrMo alloys and stainless steels, protein adsorption was potential dependent

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