Adsorption of antibiotic, heavy metal and antibiotic plasmid by a wet-state silicon-rich biochar/ferrihydrite composite to inhibit antibiotic resistance gene proliferation/transformation

Abstract

Decreasing bioaccessible antibiotics, heavy metals, and antibiotic resistance genes (ARGs) in soil by adsorption is an attractive, but unrealized, approach for ARG risk reduction. This approach has the potential to reduce the (co)selection pressure from antibiotics and heavy metals on bacteria and ARG horizontal gene transformation to pathogens. Here, a wet-state silicon-rich biochar/ferrihydrite composite (SiC–Fe(W)) synthesized by loading ferrihydrite onto rice straw-derived biochar was examined for i) adsorption of oxytetracycline and Cu2+ to reduce (co)selection pressure and ii) adsorption of extracellular antibiotic resistance plasmid pBR322 (containing tetA and blaTEM-1) to inhibit ARG transformation. SiC–Fe(W) gained the adsorption priority of biochar (for Cu2+) and wet-state ferrihydrite (for oxytetracycline and pBR322) and showed adsorptive enhancement (for Cu2+ and oxytetracycline) from a more wrinkled and exposed surface from biochar silica-dispersed ferrihydrite and a more negatively charged biochar, and the adsorption capacity for SiC–Fe(W) was 17–135 times that of soil. Correspondingly, 10 g/kg SiC–Fe(W) amendment increased the soil adsorption coefficient Kd by 31%–1417% and reduced the selection pressure from dissolved oxytetracycline, co-selection pressure from dissolved Cu2+, and transformation frequency of pBR322 (assessed with Escherichia coli). The development of Fe–O–Si bonds on silicon-rich biochar in alkaline enhanced ferrihydrite stability and adsorption capacity (for oxytetracycline), presenting a new potential strategy of biochar/ferrihydrite composite synthesis for adsorptive inhibition of ARG proliferation and transformation in ARG pollution control.