Abstract
The adsorption kinetics of laccase immobilization on magnetically-separable hierarchically-ordered mesocellular mesoporous silica (M-HMMS) as the support was studied. Past studies on the immobilization of laccase through adsorption have gradually increased, but the kinetics and mechanism of laccase adsorption on magnetic support have been poorly investigated, specifically on M-HMMS. Thus, the adsorption process was analyzed to understand the distribution and nature of laccase molecules on the M-HMMS and the rate-limiting step based on the intraparticle diffusion model. The adsorption kinetics of laccase corresponded to the pseudo-second-order and exhibited two stages of intraparticle diffusion. This study clearly indicates that the immobilization of laccase on M-HMMS is chemical adsorption with a strong covalent bond and that chemisorbed types are difficult to remove from the solid surface due to the greater bond strength in chemisorption. These results provide a deeper knowledge of the adsorption mechanisms of laccase on M-HMMS for better future applications.
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