Abstract
The adsorption of Bovine Serum Albumin (BSA) on gold and titanium surfaces in Hanks’ solution was monitored using the electrochemical quartz crystal microbalance (EQCM) technique. The changes in mass and open circuit potential at rest potential and mass and current at constant potentials were measured before and after the introduction of BSA.The mass of BSA adsorbed on titanium reached a steady value within one hour, while the albumin adsorbed on gold continued to increase. The amount of BSA adsorbed by titanium increased with concentration and stabilized at 0.8 mg/ml while that on gold did not stabilize. This indicates a monolayer of the protein on titanium and multi-layer on gold. The adsorption of BSA on gold and titanium was accelerated by the charge of potential.The time before stabilizing the adsorption‒desorption phenomena also increased with the BSA concentration.
Highlights
The interaction between metal surfaces and tissues is a complex matter
We have recently reported the characterization of glycine on gold and titanium using the electrochemical quartz crystal microbalance (EQCM) technique [10]
We report the study of the adsorption of Bovine Serum Albumin (BSA) in Hanks’ solution, on gold and titanium as characterized using the EQCM technique
Summary
The interaction between metal surfaces and tissues is a complex matter. The precise nature of the adsorption of proteins onto these artificial surfaces (e.g., titanium and gold) by the host system is important.The initial events that occur upon contact between the metals and tissues are important and may themselves have an influence on subsequent events [1]. The precise nature of the adsorption of proteins onto these artificial surfaces (e.g., titanium and gold) by the host system is important. The understanding of the adsorption and cooperative mechanisms of amino acids and proteins on surfaces is of importance in research of biomaterials [1,2] and cell adhesion [3]. Surgical metal implants in humans were primarily developed to function as prosthetic devices either for the replacement of damaged parts that cannot be replaced through treatment or fixation devices for holding bone parts in place until healing is established. The disadvantage of inadequate strength and inertness exhibited by earlier materials has resulted in the adoption of titanium and titanium alloys, stainless steel AISI Type 316L (SS 316L) and cobalt–chromium–molybdenum (Co–Cr–Mo) alloy for implants in man [4,5,6]
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