Abstract
The adsorptive behaviour of four supports in reversed-phase thin-layer chromatography was investigated. Oleyl alcohol was used as the stationary phase and methanol-water mixtures as the mobile phase. A series of 4-hydroxycoumarins were used as test compounds. Kieselguhr and cellulose show adsorption. Kieselgel also is not a good support, probably because of its large specific surface area. Kieselguhr modified by silanization behaves as a support without adsorptive sites. The R M values obtained with silanized Kieselguhr as the support correlate very well with the partition coefficients for the octanol-water system.
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