Adsorption behaviors of poly( N-p-vinylbenzyl-4- o-β- d-galactopyranosyl-[1→4]- d-gluconamide) by quartz-crystal microbalance

Abstract

Adsorption behaviors of amphiphilic poly( N-p-vinylbenzyl-4- o-β- d-galactopyranosyl-[1 → 4]- d-gluconamide) (PVLA) on the polystyrene (PS) surface was studied using 27 MHz quartz-crystal microbalance (QCM). The amount of adsorbed PVLA on PS surface was increased with an increase of PVLA concentration as a Langmuir-type in a monolayer. The saturated mass change (Δ M max) and association constant ( K a) of PVLA on PS surface were 498.6 ng/cm 2 and 1.93 × 10 7 M −1, respectively. The adsorbed PVLA on PS surface was specifically recognized by Allo A lectin due to specific interaction between galactose moieties in the PVLA and Allo A. The hydrophobic interaction between hydrophobic main chain of PVLA and hydrophobic surface of PS was reduced in the presence of urea and the diameter of PVLA aqueous solution was decreased with an increase of urea concentration.